Monitoring of phytocannabinoids in hemp seeds and their products
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F22%3A43925475" target="_blank" >RIV/60461373:22330/22:43925475 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Monitoring of phytocannabinoids in hemp seeds and their products
Original language description
In the first phase of the experimental part, the content of 17 phytocannabinoids was determined in 10 samples of hemp seeds and 10 samples of hemp oil available on the Czech market. Hemp seeds were homogenized, weighed, and an appropriate amount of dichloromethane-methanol was added. The suspension was shaken, filtered, and collected for analysis. A mixture of deuterated internal standards was added to the sample taken in this way. Quantification was performed using an external matrix calibration where the matrix was without phytocannabinoids. In the case of hemp oils, only the weighed sample was dissolved in ethanol. The sample prepared this way was also taken for analysis with the addition of a mixture of internal deuterated standards. Quantification was performed using an external solvent calibration. The analysis was carried out using the technique of reverse-phase ultra-high performance liquid chromatography coupled with tandem high-resolution mass spectrometry (UHPLC-HRMS/MS). Electrospray (ESI) operated both in positive and negative mode and was used for ionization. A quadrupole-orbitrap was used as a mass analyzer. The identification of the analyzed analytes was carried out on the basis of matching the retention times with the retention times of the analytical standards, then using the exact mass m/z of the ions of the analytes and the analytes isotopic.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10700 - Other natural sciences
Result continuities
Project
<a href="/en/project/LM2018100" target="_blank" >LM2018100: Infrastructure for Promoting Metrology in Food and Nutrition in the Czech Republic</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů