Analysis of phytocannabinoids in plant oils: benefits provided by supercritical fluid chromatography (SFC) coupled to high resolution mass spectrometry (HRMS)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F19%3A43919208" target="_blank" >RIV/60461373:22330/19:43919208 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Analysis of phytocannabinoids in plant oils: benefits provided by supercritical fluid chromatography (SFC) coupled to high resolution mass spectrometry (HRMS)

Original language description

A fast supercritical fluid chromatography – high resolution mass spectrometry based method (SFCHRMS/MS) for the determination of 17 phytocannabinoids in plant oils has been developed and validated. Analysis of phytocannabinoids in oils is a challenging task not only due to a difficult matrix, but also because of a structural similarity and high concentration variability of the target analytes. Prior the instrumental analysis, samples were only dissolved in hexane and then separated on a normal phase Acquity UPC2 Torus DIOL column using 5 mM ammonium formate and 0,1% formic acid in methanol:water (95:5, v/v) as mobile phases modifier; mass spectrometer with Q-TOF mass analyzer was used as the detector. The repeatabilities of measurements were in the range 4 – 14 % and the limits of quantitation ranged from 0.25 to 1.0 mg/kg for all analytes/oil matrix combinations. These results were comparable to those obtained by the commonly used reverse phase UHPLCHRMS/MS based methods, nevertheless, thanks to a low lipids retention under SFC conditions used, the sample analysis time was significantly reduced. Great attention was also paid to the evaluation of matrix effects as significant differences were found among plant oils (rapeseed, olive, hemp and linseed oils were analyzed). To compensate the matrix effects, deuterated internal standards of cannabidiol (CBD), Δ9-tetrahydrocannabinol (Δ9-THC) and cannabinol (CBN) were used.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů