Stereochemistry of phosphatidylglycerols from thermotolerant bacteria isolated thermal springs
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F25%3A43932149" target="_blank" >RIV/60461373:22330/25:43932149 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1016/j.chroma.2024.465517" target="_blank" >https://doi.org/10.1016/j.chroma.2024.465517</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chroma.2024.465517" target="_blank" >10.1016/j.chroma.2024.465517</a>
Alternative languages
Result language
angličtina
Original language name
Stereochemistry of phosphatidylglycerols from thermotolerant bacteria isolated thermal springs
Original language description
Phosphatidylglycerol (1,2-diacyl-sn-glycero-3-phospho-glycerol) (PG) is one of the most abundant lipids in biological membranes. However, the chirality of the carbon atom in glycerol phosphate differs among the three kingdoms: bacteria, archaea, and eukaryotes. It is commonly assumed that archaea, as well as bacteria and eukaryotes, produce only one isomer of PG. Archaeal membranes consist of phospholipids with glycerol-1phosphate in the S configuration, while the phospholipids of the other two kingdoms contain glycerol-3phosphate with (R) stereochemistry. Another chiral atom is found in glycerol with non-esterified hydroxy groups. Considering the high temperatures that accompanied the origin of life on Earth, it becomes obvious that it is necessary to clarify the importance of membrane lipids in early evolutionary times. To reconstruct the effect of high temperatures on membrane lipids, it is ideal to use microorganisms originating from a thermophilic environment analogous to the early Earth, such as the thermal groundwater of the famous spa town of Karlovy Vary. Here, we prepared all four isomers of PG, i.e., (R,S, R,R, S,R), and (S,S), by organic synthesis and analyzed the representation of individual molecular species in seven bacteria isolated from the Karlovy Vary thermal springs using chiral chromatography - mass spectrometry. Our results provide evidence that five of these strains produce all four isomers of PG and that this production is highly dependent on the cultivation temperature. Subsequent analysis by chiral chromatography revealed that the ratio of isomers, enantiomers, and diastereoisomers depends on the cultivation temperature of individual strains.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10609 - Biochemical research methods
Result continuities
Project
<a href="/en/project/EH22_008%2F0004597" target="_blank" >EH22_008/0004597: Talking microbes - understanding microbial interactions within One Health framework</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
JOURNAL OF CHROMATOGRAPHY A
ISSN
0021-9673
e-ISSN
1873-3778
Volume of the periodical
1739
Issue of the periodical within the volume
JAN 4 2025
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
9
Pages from-to
nestránkováno
UT code for WoS article
001361812700001
EID of the result in the Scopus database
2-s2.0-85209551285