Stereochemistry of Phosphatidylglycerols from Thermotolerant Bacteria Isolated from Thermal Springs

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F25%3A00601447" target="_blank" >RIV/61388971:_____/25:00601447 - isvavai.cz</a>

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0021967324008914?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0021967324008914?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2024.465517" target="_blank" >10.1016/j.chroma.2024.465517</a>

Result language

angličtina

Original language name

Stereochemistry of Phosphatidylglycerols from Thermotolerant Bacteria Isolated from Thermal Springs

Original language description

Phosphatidylglycerol (1,2-diacyl-sn-glycero-3-phospho-glycerol) (PG) is one of the most abundant lipids in biological membranes. However, the chirality of the carbon atom in glycerol phosphate differs among the three kingdoms: bacteria, archaea, and eukaryotes. It is commonly assumed that archaea, as well as bacteria and eukaryotes, produce only one isomer of PG. Archaeal membranes consist of phospholipids with glycerol-1-phosphate in the S configuration, while the phospholipids of the other two kingdoms contain glycerol-3-phosphate with (R) stereochemistry. Another chiral atom is found in glycerol with non-esterified hydroxy groups. Considering the high temperatures that accompanied the origin of life on Earth, it becomes obvious that it is necessary to clarify the importance of membrane lipids in early evolutionary times. To reconstruct the effect of high temperatures on membrane lipids, it is ideal to use microorganisms originating from a thermophilic environment analogous to the early Earth, such as the thermal groundwater of the famous spa town of Karlovy Vary. Here, we prepared all four isomers of PG, i.e., (R,S, R,R, S,R), and (S,S), by organic synthesis and analyzed the representation of individual molecular species in seven bacteria isolated from the Karlovy Vary thermal springs using chiral chromatography - mass spectrometry. Our results provide evidence that five of these strains produce all four isomers of PG and that this production is highly dependent on the cultivation temperature. Subsequent analysis by chiral chromatography revealed that the ratio of isomers, enantiomers, and diastereoisomers depends on the cultivation temperature of individual strains.

Czech name

—

Czech description

—

Type

J_ost - Miscellaneous article in a specialist periodical

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

<a href="/en/project/EH22_008%2F0004597" target="_blank" >EH22_008/0004597: Talking microbes - understanding microbial interactions within One Health framework</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

1873-3778

Volume of the periodical

1739

Issue of the periodical within the volume

January 4

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

9

Pages from-to

465517

UT code for WoS article

001361812700001

EID of the result in the Scopus database

2-s2.0-85209551285