Extraction optimization of Citrus medica L. whole fruit: phytochemical profile, pectin recovery and antioxidant activity in Caco-2 cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F25%3A43932398" target="_blank" >RIV/60461373:22330/25:43932398 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S2772502225006018?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2772502225006018?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.afres.2025.101296" target="_blank" >10.1016/j.afres.2025.101296</a>
Alternative languages
Result language
angličtina
Original language name
Extraction optimization of Citrus medica L. whole fruit: phytochemical profile, pectin recovery and antioxidant activity in Caco-2 cells
Original language description
This study aims to increase the antioxidant yield from fresh whole fruits of Citrus medica cv. Diamante Liscia applying the Full Factorial Design (FFD), which allowed to obtain an optimized extract (OE). The optimal extraction conditions obtained by FFD were 60 min, 85.30 % EtOH/H2O and 47.22 degrees C, reporting the following data: 3.79+0.09 mgGAE/g, 4.45+0.03 mgTE/g and 10.62+0.26 mgTE/g for Total Polyphenol Content (TPC), 2,2-diphenyl-1-picryl hydrazyl (DPPH) and Ferric Reducing Antioxidant Power (FRAP), respectively. To provide a comprehensive metabolic profile of samples prepared as part of the experimental design, LC-HRMS-based untargeted analysis was performed using a combination of MS-DIAL, MS-CLEANR, and MS-FINDER software suites. Pearson correlation established the relationship between antioxidant activity evaluated by TPC, DPPH, and FRAP and peaks area of specific metabolites. Of these, rutin, hesperidin, and diosmin were confirmed and quantified as OE's characteristic component. Furthermore, after recovering bioactive compounds of interest, pectin with a high yield of 14.78 + 1.26 % was recovered from the exhausted material. The OE showed antioxidant properties on intestinal Caco-2 cells by stimulating antioxidant enzymes such as superoxide dismutase, glutathione peroxidase, catalase, and NAD(P)H dehydrogenase. This study reports for the first time the antioxidant properties of C. medica whole fruit extract highlighting its potential as therapeutic agent in preventing oxidative stress-related diseases, such as inflammatory bowel disease.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40500 - Other agricultural sciences
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Applied Food Research
ISSN
2772-5022
e-ISSN
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Volume of the periodical
5
Issue of the periodical within the volume
2
Country of publishing house
IE - IRELAND
Number of pages
14
Pages from-to
nestránkováno
UT code for WoS article
001567924500006
EID of the result in the Scopus database
2-s2.0-105014775183