Open tubular capillary electrochromatgoraphy of underivatized amino acids using Rh/III tetrakis(phenoxyphenyl)porphrinate as wall modifier

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F03%3A00007431" target="_blank" >RIV/60461373:22340/03:00007431 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Open tubular capillary electrochromatgoraphy of underivatized amino acids using Rh/III tetrakis(phenoxyphenyl)porphrinate as wall modifier

Original language description

The separation of 17 "common" underivatized amino acids was attempted by open tutular capillary electrochromatography (OT.CEC) in fused-silica capillaries coated with Rh(III) tetrakis(phenoxyphenyl)porphyrinate (Rh(III)TPP(m-OPh)4OAc) using sodium phosphate and Tris-phosphate buffers as background electrolytes (BGEs). The OT-CEC separation of amino acids was compared with that obtained by capillary zone electrophoresis in bare fused-silica capilaries using the same BGEs. The amono acids were not derivatized and the UV-absorption detection was set at 200 nm. Depending on the experimental condition at least 15 amono acids were separated. The best separations were obtained in a Rh(III)TPP(m-OPh)4OAc-coated capilllary in 50 mM Tris-100 mM phosphate bufferat pH 2.25. Separation of the critical triplet val-Ile-Leu was always at least indicated being better at higher BGE concentrations. Regarding the sensitivity of the method, Iower concentration limits of detection (LODs) inf the coated cap

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GA203%2F02%2F1467" target="_blank" >GA203/02/1467: High-performance analytical and preparative separations of biologically active peptides and proteins by capillary and free-flow electromigration methods</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

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Volume of the periodical

990

Issue of the periodical within the volume

990

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

159-167

UT code for WoS article

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EID of the result in the Scopus database

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