Covalent anionic copolymer coatings with tunable electroosmotic flow for optimization of capillary electrophoretic separations

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F22%3A00561238" target="_blank" >RIV/61388963:_____/22:00561238 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11120/22:43923786 RIV/00216208:11310/22:10446084
Result on the web
<a href="https://doi.org/10.1002/elps.202200130" target="_blank" >https://doi.org/10.1002/elps.202200130</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.202200130" target="_blank" >10.1002/elps.202200130</a>

Result language
angličtina
Original language name
Covalent anionic copolymer coatings with tunable electroosmotic flow for optimization of capillary electrophoretic separations
Original language description
We present a method for finely adjustable electroosmotic flow (EOF) velocity in cathodic direction for the optimization of separations in capillary electrophoresis. To this end, we use surface modification of the separation fused silica capillary by the covalently attached copolymer of acrylamide (AM) and 2-acrylamido-2-methyl-1-propanesulfonate (AMPS), that is, poly(AM-co-AMPS) or PAMAMPS. Coatings were formed by the in-capillary polymerization of a mixture of the neutral AM and anionic AMPS monomers premixed in various ratios in order to control the charge density of the copolymer. EOF mobility varies in the 0 to similar to 40 x 10(-9) m(2) V-1 s(-1) interval for PAMAMPS coatings ranging from 0 to 60 mol.% of charged AMPS monomer. For EOF in PAMAMPS-treated capillaries, we observed (i) a negligible dependence on pH in the 2-10 interval, (ii) a minor variance among background electrolytes (BGEs) in function of their components and (iii) its standard decrease with increasing ionic strength of the BGE. Interest in variable cathodic EOF was demonstrated by the amelioration of separation of two kinds of isomeric anionic analytes, that is, monosaccharides phosphates and helquat enantiomers, in counter-EOF mode.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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