DEVELOPMENT OF AN EXPERIMENTAL MICRODEVICE FOR DETECTION OF IGG

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F05%3A00014595" target="_blank" >RIV/60461373:22340/05:00014595 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

DEVELOPMENT OF AN EXPERIMENTAL MICRODEVICE FOR DETECTION OF IGG

Original language description

Microfluidic devices are becoming an important tool in diagnostics of diseases and analysis of biological liquids. In this work, we present our progress in development of a microchip for ultra-fast and quantitative determination of human antibodies (IgG)in an aqueous sample. The method is based on the specific biochemical interaction between IgG and the immobilized antigen (Protein A). The antibody molecules are fluorescently labeled and thus their concentration in the microdevice can be monitored by fluorescence microscope. The microdevice consists of an active polystyrene part (PS) with a thin microcappilary and a part for connection of the PS part to the sample and the washing solutions. The microcapillary has diameter between 10 and 20 microns. Use of the electrokinetic phenomena (electroosmosis) for transportation of liquids in the PS part is intensively studied. All step of the immunoassay are described and the experimental determination of IgG concentration in a sample by means

Czech name

Vývoj experimentálního mikrozařízení pro detekci IgG

Czech description

V této práci je prezentován náš pokrok ve vývoji mikročipů pro ultrarychlé a kvantitativní stanovení lidských protilátek ve vodném roztoku.Jsou prezentovány všechny kroky výroby mikrofluidního zařízení a jednotlivé kroky imunoanalýzy. Jsou diskutovány možné výhody I nevýhody použitého mikročipu.

Type

D - Article in proceedings

CEP classification

CI - Industrial chemistry and chemical engineering

OECD FORD branch

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Project

<a href="/en/project/GP104%2F03%2FD005" target="_blank" >GP104/03/D005: Transport phenomena around the acid-base boundaries in spatially micro- and nano-structurized systems under the influence of an external electric field</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Proc. of 32th International Conference of Slovak Society of Chemical Engineering.

ISBN

80-227-2224-3

ISSN

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e-ISSN

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Number of pages

7

Pages from-to

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Publisher name

Slovak University of Technology

Place of publication

Bratislava

Event location

Tatranské Matliare, Slovensko

Event date

May 23, 2005

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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