Avidity of antineurocytoskeletal antibodies in Alzheimer's disease patients
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F17%3A10361459" target="_blank" >RIV/00216208:11110/17:10361459 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11120/17:43913179 RIV/00064173:_____/17:N0000172 RIV/00023752:_____/17:43919218 RIV/00064165:_____/17:10361459
Result on the web
<a href="http://dx.doi.org/10.5507/bp.2017.017" target="_blank" >http://dx.doi.org/10.5507/bp.2017.017</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.5507/bp.2017.017" target="_blank" >10.5507/bp.2017.017</a>
Alternative languages
Result language
angličtina
Original language name
Avidity of antineurocytoskeletal antibodies in Alzheimer's disease patients
Original language description
Aims. To optimise the ELISA method for the avidity of IgG antibodies against neurofilament heavy chain (NfH) and to determine the levels and avidity of anti-NfH antibodies in patients with Alzheimer's disease (AD) and a healthy control group. Methods. Various dilutions of sera and concentrations of urea and sodium chloride as chaotropic reagents were tested in the process of the ELISA optimisation. The levels and avidity of anti-NfH antibodies were determined in 30 patients with Alzheimer's disease and 30 age-matched cognitively normal elderly adults. Results. Sera dilution 1: 200 and urea as a chaotrope in a concentration 6 mol/L were chosen to be the most suitable for the avidity assay of anti-NfH antibodies by ELISA. The results showed no differences in either level or avidity of IgG anti-NfH antibodies between AD patients and cognitively normal persons. The levels of anti-NfH IgG antibodies inversely correlated with their avidities.Conclusions. We optimised the ELISA method for the determination of anti-NfH antibody avidity determination which is suitable for research of anti-NfH antibody avidity in patients with neurological diseases associated with neurocytoskeletal defects. The determination of serum anti-NfH antibody avidity in AD patients seems to have limited diagnostic significance.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30103 - Neurosciences (including psychophysiology)
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biomedical Papers
ISSN
1213-8118
e-ISSN
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Volume of the periodical
161
Issue of the periodical within the volume
2
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
8
Pages from-to
179-186
UT code for WoS article
000406522700009
EID of the result in the Scopus database
2-s2.0-85020548974