Comparison of different enzyme-linked immunosorbent assay methods for avidity determination of antiphospholipid antibodies
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F17%3A10365784" target="_blank" >RIV/00216208:11110/17:10365784 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/jcla.22121" target="_blank" >http://dx.doi.org/10.1002/jcla.22121</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jcla.22121" target="_blank" >10.1002/jcla.22121</a>
Alternative languages
Result language
angličtina
Original language name
Comparison of different enzyme-linked immunosorbent assay methods for avidity determination of antiphospholipid antibodies
Original language description
Background: Avidity of antiphospholipid antibodies may be clinically useful as a valuable additional characteristic. The aim of this study was to compare several ELISA modifications with different chaotropic agents and calculation of avidity indices for the determination of anticardiolipin antibody (aCL) avidity. Methods: We examined 28 serum samples with positive IgG aCL by adapted ELISA using various concentrations of urea and sodium chloride as chaotropic agents and different dilution of sera. We tested these conditions of ELISA-a single diluted serum sample with fixed concentration of a chaotrope and a serially diluted serum in the constant concentration of a chaotropic agent. Results: We demonstrated that ELISA method for avidity determination based on a single dilution of serum in the presence of fixed concentration of chaotrope is convenient for determination of IgG aCL antibody avidity. Concentrations 6 and 8 mol/L of urea or 1 and 2 mol/L of NaCl were suitable for sufficient dissociation of immune complexes during ELISA procedure. Conclusion: This way was in good agreement with more demanding procedures. Both urea and sodium chloride may be used as chaotropic agents. Reference values of avidity indices essential for interpretation of patients' results must be established individually for distinct assay conditions.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Clinical Laboratory Analysis
ISSN
0887-8013
e-ISSN
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Volume of the periodical
31
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
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UT code for WoS article
000416049100010
EID of the result in the Scopus database
2-s2.0-85011397544