Avidity of anticardiolipin antibodies-A factor that could be important for their detection by ELISA methods

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F14%3A10283475" target="_blank" >RIV/00216208:11110/14:10283475 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.jab.2014.01.005" target="_blank" >http://dx.doi.org/10.1016/j.jab.2014.01.005</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jab.2014.01.005" target="_blank" >10.1016/j.jab.2014.01.005</a>

Result language

angličtina

Original language name

Avidity of anticardiolipin antibodies-A factor that could be important for their detection by ELISA methods

Original language description

Avidity is an important feature of antibodies associated with their pathogenic effects. Anticardiolipin antibodies (aCLs) are the most commonly examined antiphospholipid antibodies, however, their avidity has been investigated only marginally. The aim ofthe study was to compare the avidity of the antibodies specifically bound to the cardiolipin-coated microtitrate wells with those bound to the wells bearing no cardiolipin in various conditions. We analysed 22 serum samples with high, medium and low levels of aCL IgG. The avidity of aCL IgG was determined in serially diluted sera by the ELISA method in the presence of increasing urea concentrations (from 2 to 8 mol/L) as a chaotropic agent. The serum dilution 1:50 and 1:100 and the concentrations of urea 6 mol/L and 8 mol/L seemed to be suitable for the determination of aCL avidity. The avidity of antibodies specifically bound to the cardiolipin and those bound to the cardiolipin-free wells significantly differed in their avidity. The

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EC - Immunology

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Applied Biomedicine

ISSN

1214-021X

e-ISSN

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Volume of the periodical

12

Issue of the periodical within the volume

4

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

8

Pages from-to

277-284

UT code for WoS article

000345065200012

EID of the result in the Scopus database

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