Modeling reaction-transport processes in a microcapillary biosensor for detection of human IgG

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F06%3A00016652" target="_blank" >RIV/60461373:22340/06:00016652 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Modeling reaction-transport processes in a microcapillary biosensor for detection of human IgG

Original language description

Mathematical modeling of electrokinetic transport processes and antigen-antibody interactions in a microfluidic biosensor for heterogeneous immunoassay is described. Two mathematical models of externally controlled electroosmotic transport are constructed - the slip model based on Helmholtz-Smoluchowski approximation and the non-slip model based on the use of Poisson equation. It was found that the use of the slip model is restricted by concentration of the carrier electrolyte and dimensions of the system. The antigen-antibody interaction cannot be described by the slip model. Dynamics of the heterogeneous immunoassay in the proposed biosensor was also studied. Human-IgG-Protein A was used as model system. Parametric studies revealed strong sensitivityof the biosensor to the antibody charge number and to the character of surface heterogeneity in the position of immobilized antigen. This sensitivity can be one of the reasons why the practical applications of heterogeneous protein chips

Czech name

Modelování reakčně transportních procesů v mikrokapilárním biosenzoru pro detekci lidských IgG

Czech description

Matematické modelování elektrokinetických transportních procesů a interakce antigen-protilátka v mikrofluidním biosenzoru pro heterogenní imunoanalýzu jsou popsány. Dva matematické modely externě řízeného elektrokinetického transportu jsou vytvořeny - slip model založený na Helmholtz-Smoluchowského aproximaci a neskluzový model založený na Poissonově rovnici. Parametrické studie odhalily velkou citlivost biosenzoru k nábojovému číslu protilátky a charakteru povrchové heterogenity v místě imobilizovanéhoantigenu.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CI - Industrial chemistry and chemical engineering

OECD FORD branch

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Project

<a href="/en/project/GP104%2F03%2FD006" target="_blank" >GP104/03/D006: Study of microreactors for bioengineering application</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Microelectronic Engineering

ISSN

0167-9317

e-ISSN

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Volume of the periodical

83

Issue of the periodical within the volume

4-9

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

4

Pages from-to

1660-1663

UT code for WoS article

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EID of the result in the Scopus database

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