Immunoanalytical detection of bioaffinity complexes in PDMS microfluidic chips

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F09%3A00021985" target="_blank" >RIV/60461373:22340/09:00021985 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Immunoanalytical detection of bioaffinity complexes in PDMS microfluidic chips
Original language description
In the experiments, the biological receptor is immobilized on the microchannel walls by a passive sorption. The blocking reagent 1 % BSA is used to cover empty regions on the walls. Then, aqueous solutions of ligands and detection reagents with a fluorescent marker are subsequently introduced in the channels. Finally, a solution of a detergent is used to break non-specific bonds among the biomolecules. The main advantage of the used experimental arrangement is that the reaction time of each reaction step in this experimental arrangement is not longer than few minutes due to the small characteristic dimensions.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Sborník prací ze semináře Reakční a transportní jevy III
ISBN
978-80-7080-725-5
ISSN
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e-ISSN
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Number of pages
9
Pages from-to
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Publisher name
VŠCHT Praha
Place of publication
Praha
Event location
Jablonec nad Nisou
Event date
Jun 10, 2009
Type of event by nationality
CST - Celostátní akce
UT code for WoS article
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