Detection of bioaffinity complexes in microchannels by fluorescence techniques.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F08%3A00020219" target="_blank" >RIV/60461373:22340/08:00020219 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Detection of bioaffinity complexes in microchannels by fluorescence techniques.
Original language description
This work deals with multichannel microfluidic systems intended for bioapplications, particularly for medical diagnostics. Microfluidic systems consist of several parallel channels with small characteristic dimensions (10-100 ?m). These devices are fabricated from polystyrene (PS) and polydimethylsiloxane (PDMS) by a combination of microfabrication techniques ? UV photolithography, micromilling, thermal bonding, galvanic metallization, casting etc. In the experiments, the biological receptor is immobilized on the microchannel walls by a passive sorption. The blocking reagent 1 % BSA is used to cover empty regions on the walls. Then, aqueous solutions of ligands and detection reagents with a fluorescent marker are subsequently introduced in the channels. Finally, a solution of a detergent is used to break nonspecific bonds among the biomolecules. The main advantage of the used experimental arrangement is that the reaction time of each reaction step in this experimental arrangement is no
Czech name
Detekce bioafinitních komplexů v mikrokanále fluorescenčními technikami
Czech description
Práce je zaměřena na výzkum vícekanaálového mikrofluidního zařízení pro bioanalýzu. Je posána technika přípravy mkročipu z PDMS. Je navržena sendvičová metoda stanovení feritinu v mikrokanálku a jsou uskutečněny validační experimenty.
Classification
Type
D - Article in proceedings
CEP classification
CI - Industrial chemistry and chemical engineering
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
CD-ROM of Full Texts CHISA 2008
ISBN
978-80-02-02047-9
ISSN
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e-ISSN
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Number of pages
8
Pages from-to
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Publisher name
Process Engineering Publisher
Place of publication
Praha
Event location
Praha
Event date
Aug 24, 2008
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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