Enzyme synthesis of cephalexin in continuous-flow microfluidic device in ATPS environment

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F20%3A43921138" target="_blank" >RIV/60461373:22340/20:43921138 - isvavai.cz</a>

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S1385894720312286?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1385894720312286?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cej.2020.125236" target="_blank" >10.1016/j.cej.2020.125236</a>

Result language

angličtina

Original language name

Enzyme synthesis of cephalexin in continuous-flow microfluidic device in ATPS environment

Original language description

We present an experimental study dealing with the transfer of the enzyme synthesis of cephalexin from a batch arrangement to a continuous-flow microfluidic system with integrated reaction product separation and enzyme recovery. Cephalexin is synthesized by penicillin acylase in a kinetic regime that is characterized by the appearance of a concentration maximum during the enzyme reaction. We determine proper reaction conditions providing a high cephalexin yield and relatively short reaction time by performing a set of batch experiments. Once the residence/reaction time was found, the reaction process is transferred into a continuous mode. We employ an aqueous two-phase system (ATPS) forming two-phase slug flow in a microfluidic capillary as the reaction-separation environment. Such a flow arrangement guarantees a uniform residence time of the reaction mixture in the reaction microcapillary, and it provides in situ extraction of cephalexin. ATPS also offers an easy way for enzyme recycling and addition of fresh reactants as a benefit. We optimized the composition of the ATPS based on phosphates, polyethylene glycol and water so that cephalexin showed a high affinity to one phase and a soluble enzyme to the other phase. Due to the low level of utilization of a soluble catalyst in the continuous flow arrangement, we recycled the reaction phase containing the dissolved enzyme. The recycling loop also contained microdialysis unit for removal of phenylglycine that tends to cause system clogging. We show that the final design of the microfluidic system with recycle can operate continuously for at least 5 h. Our integrated microfluidic platform represents a general solution for enzymatic reactions performed with simultaneous reaction product separation and enzyme recycle.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

20402 - Chemical process engineering

Project

<a href="/en/project/GA17-09914S" target="_blank" >GA17-09914S: Reaction-transport fundamentals in integrated microfluidic bioreactors-separators operating with aqueous two-phase systems</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemical Engineering Journal

ISSN

1385-8947

e-ISSN

—

Volume of the periodical

396

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

"125236-1"-"125236-11"

UT code for WoS article

000551969200064

EID of the result in the Scopus database

2-s2.0-85083865441