Electric-field-enhanced selective separation of products of an enzymatic reaction in a membrane micro-contactor
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F21%3A43923007" target="_blank" >RIV/60461373:22340/21:43923007 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/10.1002/bit.27597" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1002/bit.27597</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/bit.27597" target="_blank" >10.1002/bit.27597</a>
Alternative languages
Result language
angličtina
Original language name
Electric-field-enhanced selective separation of products of an enzymatic reaction in a membrane micro-contactor
Original language description
Processes employed in separations of products of enzyme reactions are often driven by diffusion, and their efficiency can be limited. Here, we exploit the effect of a direct current (DC) electric field that intensifies mass transfer through a semipermeable membrane for fast, continuous, and selective separation of electrically charged molecules. Specifically, we separate low-molecular-weight reaction products (phenylacetic acid, 6-aminopenicillanic acid) from the original reaction mixture containing a free enzyme (penicillin acylase). The developed microfluidic dialysis-membrane contactor allows a stable counter-current arrangement of the retentate and permeates liquid streams on which DC electric field is perpendicularly applied. The applied electric field significantly accelerates the transport of electrically charged products through the semipermeable membrane yielding high separation efficiencies at short residence times. The residence time of 5 min is sufficient to reach 100% separation yield in the electric field. The same residence time provides only a 50% yield in the diffusion-controlled experiments. We experimentally demonstrated that a combined microreactor–microextractor with a recycle of the soluble penicillin acylase can continuously produce both the reaction products at high concentrations. The developed membrane-contactor is a versatile platform allowing to tune its characteristics, such as selectivity given by the membrane, or the type of the retentate phase, for a specific application. © 2020 Wiley Periodicals LLC
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
20402 - Chemical process engineering
Result continuities
Project
<a href="/en/project/GA20-09980S" target="_blank" >GA20-09980S: Microfluidic reactors with embedded semi-permeable membranes for the preparation of special chemicals</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biotechnology and Bioengineering
ISSN
0006-3592
e-ISSN
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Volume of the periodical
118
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
715-724
UT code for WoS article
000581953200001
EID of the result in the Scopus database
2-s2.0-85093520479