Gold and silver nanoparticles for biomolecule immobilization and enzymatic catalysis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F12%3A43893382" target="_blank" >RIV/60461373:22340/12:43893382 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1186/1556-276X-7-287" target="_blank" >http://dx.doi.org/10.1186/1556-276X-7-287</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/1556-276X-7-287" target="_blank" >10.1186/1556-276X-7-287</a>

Result language

angličtina

Original language name

Gold and silver nanoparticles for biomolecule immobilization and enzymatic catalysis

Original language description

In this work, a simple method for alcohol synthesis with high enantiomeric purity was proposed. For this, colloidal gold and silver surface modifications with 3-mercaptopropanoic acid and cysteamine were used to generate carboxyl and amine functionalizedgold and silver nanoparticles of 15 and 45 nm, respectively. Alcohol dehydrogenase from Thermoanaerobium brockii (TbADH) and its cofactor (NADPH) were physical and covalent (through direct adsorption and using cross-linker) immobilized on nanoparticles'surface. In contrast to the physical and covalent immobilizations that led to a loss of 90% of the initial enzyme activity and 98% immobilization, the use of a cross-linker in immobilization process promoted a loss to 30% of the initial enzyme activityand } 92% immobilization. The yield of NADPH immobilization was about 80%. The best results in terms of activity were obtained with Ag-citr nanoparticle functionalized with carboxyl groups (Ag-COOH), Au-COOH(CTAB), and Au-citr functionali

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nanoscale Research Letters

ISSN

1931-7573

e-ISSN

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Volume of the periodical

7

Issue of the periodical within the volume

287

Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

1-10

UT code for WoS article

000309228200001

EID of the result in the Scopus database

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