Effect of cross-linking method on the activity of spray-dried chitosan microparticles with immobilized laccase

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F13%3A43895409" target="_blank" >RIV/60461373:22340/13:43895409 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.fbp.2013.06.001" target="_blank" >http://dx.doi.org/10.1016/j.fbp.2013.06.001</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.fbp.2013.06.001" target="_blank" >10.1016/j.fbp.2013.06.001</a>

Result language
angličtina
Original language name
Effect of cross-linking method on the activity of spray-dried chitosan microparticles with immobilized laccase
Original language description
The effect of processing method on the properties of cross-linked chitosan microparticles and on the enzymatic activity of laccase immobilized in the particles has been investigated. Chitosan has been cross-linked by tri-polyphosphate (TPP) using two methods ? the so called ex situ cross-linking whereby the solutions of chitosan, TPP and the enzyme have been pre-mixed and spray-dried by a standard two-fluid kinetic nozzle, and a novel in situ cross-linking method, whereby the solutions have been contacted at the tip of a three-fluid nozzle and cross-linking occurred within a drying droplet. The influence of the cross-linking method on the particle size and morphology, surface charge, and swelling ratio has been determined. The enzymatic activity of laccase toward the oxidation of a chromophore substrate (ABTS) has been systematically investigated and found to be superior in particles produced by the in situ cross-linking method.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CI - Industrial chemistry and chemical engineering
OECD FORD branch
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Project
<a href="/en/project/MEB061108" target="_blank" >MEB061108: Development of chitosan based biomedical polymers with antibacterial properties</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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