An unstructured model of metabolic and temperature dependent cell cycle arrest in hybridoma batch and fed-batch cultures

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F15%3A43900187" target="_blank" >RIV/60461373:22340/15:43900187 - isvavai.cz</a>

Result on the web

<a href="http://www.sciencedirect.com/science/article/pii/S1369703X14002976" target="_blank" >http://www.sciencedirect.com/science/article/pii/S1369703X14002976</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bej.2014.10.013" target="_blank" >10.1016/j.bej.2014.10.013</a>

Result language

angličtina

Original language name

An unstructured model of metabolic and temperature dependent cell cycle arrest in hybridoma batch and fed-batch cultures

Original language description

ell productivity in fed-batch processes can be increased by cell cycle arrest through mild hypothermia. However, hypothermia can simultaneously reduce cell growth, which is regulated by the cell cycle. Consequently, the time point for the temperature shift is important and requires optimization while considering the cell cycle. An unstructured cell cycle model which includes the distribution of proliferating (G(1), S, G(2)/M) and arrested cells (G(0)) has been proposed in order to predict the time pointof temperature shift in fed-batch cultures. A model development and analysis framework that enables the evaluation of the required model parameters is described. The parameters are estimated from (fed)-batch cultivations, carried out at 37 degrees C andat 33 degrees C in order to characterize temperature dependency. The batch cultures are also used to evaluate substrate depletion and fed-batch cultures are used to study the impact of metabolite accumulation on the cell cycle. The relia

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CI - Industrial chemistry and chemical engineering

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biochemical Engineering Journal

ISSN

1369-703X

e-ISSN

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Volume of the periodical

93

Issue of the periodical within the volume

neuveden

Country of publishing house

BE - BELGIUM

Number of pages

14

Pages from-to

260-273

UT code for WoS article

000347362100032

EID of the result in the Scopus database

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