Speciation analysis of selenium in human urine by liquid chromatography and inductively coupled plasma mass spectrometry for monitoring of selenium in body fluids

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F15%3A43900396" target="_blank" >RIV/60461373:22340/15:43900396 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/0.1080/09542299.2015.1107502" target="_blank" >http://dx.doi.org/0.1080/09542299.2015.1107502</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/09542299.2015.1107502" target="_blank" >10.1080/09542299.2015.1107502</a>

Result language

angličtina

Original language name

Speciation analysis of selenium in human urine by liquid chromatography and inductively coupled plasma mass spectrometry for monitoring of selenium in body fluids

Original language description

Speciation analysis of selenium metabolites in urine was performed using hyphenation of mixed ion-pair reversed-phase chromatography and inductively coupled plasma mass spectrometry. A chromatographic separation was performed with a C8 stationary phase and a mobile phase containing 2.5 mmol LMINUS SIGN 1 sodium butane-1-sulfonate, 8 mmol LMINUS SIGN 1 tetramethylammonium hydroxide, 4 mmol LMINUS SIGN 1 malonic acid, and 0.05% methanol, pH 3.0. Under this condition, the selenium species selenite, selenate, selenomethionine, selenoethionine, selenourea, trimethylselenonium, and Se-methylselenocysteine were successfully separated. Selenium determination was carried out by monitoring 80Se. The internal standard Ge was added into the mobile phase. The calibration was linear at least up to 100 ?g LMINUS SIGN 1 Se for all species. The limit of detection was 0.4 ?g LMINUS SIGN 1 Se. When higher (1400 W) ICP power was applied, the calibration based solely on selenate provided accurate results f

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemical Speciation and Bioavailability

ISSN

0954-2299

e-ISSN

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Volume of the periodical

27

Issue of the periodical within the volume

3

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

127-138

UT code for WoS article

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EID of the result in the Scopus database

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