Microarray-based MALDI-TOF mass spectrometry enables monitoring of monoclonal antibody production in batch and perfusion cell cultures

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F16%3A43902862" target="_blank" >RIV/60461373:22340/16:43902862 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.ymeth.2015.12.011" target="_blank" >http://dx.doi.org/10.1016/j.ymeth.2015.12.011</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ymeth.2015.12.011" target="_blank" >10.1016/j.ymeth.2015.12.011</a>

Result language

angličtina

Original language name

Microarray-based MALDI-TOF mass spectrometry enables monitoring of monoclonal antibody production in batch and perfusion cell cultures

Original language description

Cell culture process monitoring in monoclonal antibody (mAb) production is essential for efficient process development and process optimization. Currently employed online, at line and offline methods for monitoring productivity as well as process reproducibility have their individual strengths and limitations. Here, we describe a matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)-based on a microarray for mass spectrometry (MAMS) technology to rapidly monitor a broad panel of analytes, including metabolites and proteins directly from the unpurified cell supernatant or from host cell culture lysates. The antibody titer is determined from the intact antibody mass spectra signal intensity relative to an internal protein standard spiked into the supernatant. The method allows a semi-quantitative determination of light and heavy chains. Intracellular mass profiles for metabolites and proteins can be used to track cellular growth and cell productivity.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CI - Industrial chemistry and chemical engineering

OECD FORD branch

—

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Methods Enzymol.

ISSN

0076-6879

e-ISSN

—

Volume of the periodical

104

Issue of the periodical within the volume

neuveden

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

33-40

UT code for WoS article

—

EID of the result in the Scopus database

—