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Immobilized strychnine as a new chiral stationary phase for HPLC

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F17%3A43913118" target="_blank" >RIV/60461373:22340/17:43913118 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216208:11310/17:10368237 RIV/00216208:11110/17:10368237

  • Result on the web

    <a href="http://onlinelibrary.wiley.com/doi/10.1002/elps.201600505/abstract;jsessionid=2A786D9B0FA42CE5055686403A6C39AC.f02t04" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/elps.201600505/abstract;jsessionid=2A786D9B0FA42CE5055686403A6C39AC.f02t04</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1002/elps.201600505" target="_blank" >10.1002/elps.201600505</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Immobilized strychnine as a new chiral stationary phase for HPLC

  • Original language description

    A new ion-exchanger type chiral stationary phase for high-performance liquid chromatography was prepared. The synthetic protocol is based on derivatization of silica with (3-iodopropyl) trimethoxysilane in the first step followed by immobilization of strychnine via quaternization of nitrogen atom of the alkaloid strychnine. The synthesized chiral stationary phase was chromatographically characterized. The main effort was headed towards the evaluation of the enantioselectivity of the novel sorbent. For that purpose a set of suitable chiral probes, specifically, binaphthyl derivatives, was employed. The influence of methanol content, concentration of aqueous ammonium acetate buffer, and its pH on retention factors, separation selectivity, and resolution of the atropoisomers of the mentioned chiral solutes was studied in detail. It was demonstrated that the new chiral stationary phase was capable to separate atropoisomers of four out of seven testing compounds. Despite the strong influence of the above mentioned variables on retention, their impact on selectivity and resolution was rather moderate. Concerning retention mechanism, it seems that electrostatic interaction between the positively charged quaternary nitrogen of the chiral stationary phase and anionic solute participates significantly in the retention process.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10406 - Analytical chemistry

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2017

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Electrophoresis

  • ISSN

    0173-0835

  • e-ISSN

  • Volume of the periodical

    38

  • Issue of the periodical within the volume

    Neuvedeno

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    8

  • Pages from-to

    1956-1963

  • UT code for WoS article

    000406724200017

  • EID of the result in the Scopus database

    2-s2.0-85019359726