Urease adsorption immobilization on ionic liquid-like macroporous polymeric support

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F19%3A43919406" target="_blank" >RIV/60461373:22340/19:43919406 - isvavai.cz</a>

Result on the web

<a href="https://link.springer.com/article/10.1007%2Fs10853-019-03980-0" target="_blank" >https://link.springer.com/article/10.1007%2Fs10853-019-03980-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10853-019-03980-0" target="_blank" >10.1007/s10853-019-03980-0</a>

Result language

angličtina

Original language name

Urease adsorption immobilization on ionic liquid-like macroporous polymeric support

Original language description

In this work, we report the synthesis and application of polymeric macroporous materials functionalized with ionic liquid (IL)-like moieties to serve as a support for enzyme immobilization. The method was based on bottom-up approach, where poly(styrene-divinylbenzene) (PS-DVB) nanoparticles were used as building blocks to form porous structures. Surface functionalization was done by introducing 1-butyl imidazole into the PS-DVB support to form IL-like imidazolium, which was consequently used for enzyme adsorption immobilization on the porous surface. To demonstrate activity of immobilized enzyme, hydrolysis of urea catalyzed by Jack bean urease was used as a model reaction. The enzymatic activity of the urease to convert urea solution into carbon dioxide (hydrocarbonates in water solution) and ammonia under acidic conditions were monitored both by measuring changes in pH and by a color change in cresol red pH indicator. The immobilized urease was able to provide hydrolysis of urea solution for 30 days while maintaining its activity over 100% compared to free enzyme solution. The activity of freshly immobilized enzyme was increased up to 285% compared to free urease solution under acidic conditions, which is caused by an acidic shift of activity-pH bell-shaped curve. Prepared porous material with immobilized urease was able to undergo four consequent cycles over the period of 4 days with only 15% decrease in activity. Overall, the results indicated that the polymeric support is well suitable to combine the advantages of macroporous material with IL-like surface moieties for enzyme immobilization and its consequent application in bio-catalytic reactions.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

20401 - Chemical engineering (plants, products)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Materials Science

ISSN

0022-2461

e-ISSN

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Volume of the periodical

54

Issue of the periodical within the volume

24

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

14884-14896

UT code for WoS article

000489150200022

EID of the result in the Scopus database

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