Spectrometric and voltammetric analysis of urease. Nickel nanoelectrode as an electrochemical sensor

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F07%3A00019452" target="_blank" >RIV/00216224:14110/07:00019452 - isvavai.cz</a>

Alternative codes found

RIV/62156489:43210/07:00112321 RIV/00216305:26220/07:PU69501 RIV/70883521:28110/07:43865398

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Spectrometric and voltammetric analysis of urease. Nickel nanoelectrode as an electrochemical sensor

Original language description

Urease is the enzyme catalyzing the hydrolysis of urea into carbon dioxide and ammonia. This enzyme is substrate-specific, which means that the enzyme catalyzes the hydrolysis of urea only. This feature is a basic diagnostic criterion used in the determination of many bacteria species. Most of the methods utilized for detection of urease are based on analysis of its enzyme activity, the hydrolysis of urea. The aim of this work was to detect urease indirectly by spectrometric method and directly by voltammetric methods. As spectrometric method we used is called indophenol assay. The sensitivity of detection itself is not sufficient to analyse the samples without pre-concentration steps. Therefore we utilized adsorptive transfer stripping technique coupled with differential pulse voltammetry to detect urease. The influence of accumulation time, pH of supporting electrolyte and concentration of urease on the enzyme peak height was investigated. Under the optimized experimental conditions

Czech name

Spektrometrická a voltametrická analýza ureázy. Niklová nanoelektroda jako elektrochemický sensor

Czech description

Ureáza je enzym katalyzující přeměnu močoviny na oxid uhličitý a amoniak. Ureáza je substrátově specifická, což znamená, že katalyzuje přeměnu pouze ureázy. Tato schopnost je základním diagnostickým kritériem používaným pro stanovení mnoha bakteriálníchdruhů. Metoda, která se využívá pro stanovení ureázy je založena na detekci její aktivity, intenzity hydrolýzy močoviny. Cílem této práce je nepřímé stanovení ureázy pomocí spektrometrických a elektrochemických metod.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CG - Electrochemistry

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Sensors

ISSN

1424-8220

e-ISSN

—

Volume of the periodical

7

Issue of the periodical within the volume

7

Country of publishing house

CH - SWITZERLAND

Number of pages

17

Pages from-to

1238-1255

UT code for WoS article

—

EID of the result in the Scopus database

—