A comprehensive study on capillary surface modifications for electrophoretic separations of liposomes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F24%3A43930881" target="_blank" >RIV/60461373:22340/24:43930881 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/24:10493022

Result on the web

<a href="https://doi.org/10.1007/s00706-024-03235-y" target="_blank" >https://doi.org/10.1007/s00706-024-03235-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00706-024-03235-y" target="_blank" >10.1007/s00706-024-03235-y</a>

Result language

angličtina

Original language name

A comprehensive study on capillary surface modifications for electrophoretic separations of liposomes

Original language description

Electroosmotic flow significantly impacts the resolution of separations in capillary electrophoresis and its modification is often necessary. Coating of the inner capillary surface either dynamically or permanently offers a way to alter the electroosmotic flow, potentially reducing the adsorption of analytes, in our case liposomes, to the capillary wall. At first, we measured fluorescently labeled liposomes in an uncoated capillary by capillary electrophoresis with laser-induced fluorescence detection. We used a special procedure of the electrophoretic experiment allowing us to observe the development of peak shape at the early stages of migration. We proved that the liposomes were adsorbed to the capillary wall, which led to a very quick and severe dispersion of their peak during their electromigration. For this reason, we used a commercially coated capillary with polyvinyl alcohol, where at the same separation conditions, we observed the peak of the liposome with a stable shape during the migration. However, this capillary is costly, thus four simple dynamic coating methods were tested for four polymers, namely Pluronic F-127, polyvinyl pyrrolidone K30, polyethylene glycol, and polydiallyldimethylammonium chloride. Among them, we chose a method where we flushed the capillary with a 10% solution of polyvinyl pyrrolidone K30 before the first measurement. In addition, in-house made permanent coating with linear polyacrylamide was investigated, which has led to effective suppression of the electroosmotic flow and stable liposome peak, not dispersed during its migration. Liposome separation using this coating fully confirmed that the studied liposomes are negatively charged and migrate in the anodic direction. Graphical abstract: (Figure presented.) © Springer-Verlag GmbH Austria, part of Springer Nature 2024.

Czech name

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Czech description

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Type

J_SC - Article in a specialist periodical, which is included in the SCOPUS database

CEP classification

—

OECD FORD branch

10403 - Physical chemistry

Project

<a href="/en/project/GA24-11986S" target="_blank" >GA24-11986S: Liposomal probes into colligative drug permeability</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

MONATSHEFTE FUR CHEMIE

ISSN

0026-9247

e-ISSN

—

Volume of the periodical

155

Issue of the periodical within the volume

8-9

Country of publishing house

DE - GERMANY

Number of pages

10

Pages from-to

835-844

UT code for WoS article

—

EID of the result in the Scopus database

2-s2.0-85199609753