Corrigendum to “Osmotic injury and cytotoxicity for hMSCs in contact with Me2SO: The effect of cell size distribution” [Cryobiology 116 (2024) 104943]
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F25%3A43932203" target="_blank" >RIV/60461373:22340/25:43932203 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0011224025000951" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0011224025000951</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.cryobiol.2025.105289" target="_blank" >10.1016/j.cryobiol.2025.105289</a>
Alternative languages
Result language
angličtina
Original language name
Corrigendum to “Osmotic injury and cytotoxicity for hMSCs in contact with Me2SO: The effect of cell size distribution” [Cryobiology 116 (2024) 104943]
Original language description
The paper discusses the impact of cell size on cytotoxicity and expansion lysis during the osmotic excursions resulting from the contact of hMSCs from UCB with Me2SO. It builds upon the mathematical model recently presented by the authors, which pertains to a population of cells with uniform size. The objective is to enhance the model's relevance by incorporating the more realistic scenario of cell size distribution, utilizing a Population Balance Equations approach. The study compares the capability of the multiple-sized model to the single-sized one to describe system behavior experimentally measured through cytofluorimetry and Coulter counter when, first, suspending hMSCs in hypertonic solutions of Me2SO (at varying osmolality, system temperature, and contact times), and then (at room temperature) pelleting by centrifugation before suspending the cells back to isotonic conditions. Simulations demonstrate that expansion lysis and cytotoxic effect are not affected by cell size for the specific system hMSCs/Me2SO, thus confirming what was found so far by the authors through a single-size model. On the other hand, simulations show that, when varying the adjustable parameters of the model that are expected to change from cell to cell lineages, expansion lysis is sensitive to cell size, while cytotoxicity is not, being mainly influenced by external CPA concentration and contact duration. More specifically, it is found that smaller cells suffer expansion lysis more than larger ones. The findings suggest that different cells from hMSCs may require a multiple-sized model to assess cell damage during osmotic excursions in cryopreservation.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
R - Projekt Ramcoveho programu EK
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
CRYOBIOLOGY
ISSN
0011-2240
e-ISSN
1090-2392
Volume of the periodical
Neuveden
Issue of the periodical within the volume
2025/08/06/
Country of publishing house
US - UNITED STATES
Number of pages
1
Pages from-to
105289
UT code for WoS article
001569303500003
EID of the result in the Scopus database
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