GC/MS determination of ibotenic acid and muscimol in the urine of patients intoxicated with Amanita pantherina

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61383082%3A_____%2F11%3A0018" target="_blank" >RIV/61383082:_____/11:0018 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s00414-011-0599-9" target="_blank" >http://dx.doi.org/10.1007/s00414-011-0599-9</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00414-011-0599-9" target="_blank" >10.1007/s00414-011-0599-9</a>

Result language
angličtina
Original language name
GC/MS determination of ibotenic acid and muscimol in the urine of patients intoxicated with Amanita pantherina
Original language description
Authors of this article introduce an instrumental method of proving Amanita pantherina or A. muscaria poisoning. The article describes the isolation of ibotenic acid and muscimol from urine, the derivatization step and the determination of these compounds by gas chromatography/mass spectrometry. Isolation of these alkaloids from urine was performed on a strong cation exchanger (Dowex? 50W X8), and the elution and derivatization of the alkaloids were made in one step with ethyl chloroformate in aqueous solution of sodium hydroxide with the addition of ethanol and pyridine. Cycloserine was used as internal standard. By this method, concentrations of ibotenic acid and muscimol in the urine of four persons intoxicated with Amanita pantherina were determined. In this study mass spectra of derivatized ibotenic acid and muscimol are shown and validation of the method is described.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FP - Other medical fields
OECD FORD branch
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Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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