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Determination of Urinary 8-hydroxy-2''-deoxyguanosine in Obese Patiens by HPLC with Electrochemical Detection

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F04%3A00101099" target="_blank" >RIV/61388955:_____/04:00101099 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Determination of Urinary 8-hydroxy-2''-deoxyguanosine in Obese Patiens by HPLC with Electrochemical Detection

  • Original language description

    A simple amperometric detector with a platinum tubular electrode was tested for the determination of urinary 8-hydroxy-2''-deoxyguanosine (8-OHdG) by high-performance liquid chromatography. Analyte was extracted from human urine using a single-step solid-phase extraction with the average recovery of 71 +/- 7%. Analysis was carried out on a C-18 analytical column under isocratic conditions. The limit of detection of 8-OHdG of 3 nM (S/N = 3) was comparable with that achieved using the conventional amperometric detector. Procedure was used for the determination of 8-OHdG levels in 10 obese patients (body-mass index > 30 kg m(-2)) yielding the mean concentration 32 +/- 27.1 nM of 8-OHdG or 1.85 +/- 0.72 .mu.mol 8-OHdG mol(-1) creatinine (4.63 +/- 1.8. mu.g8-OHdG g(-1) creatinine), which is not much different from the levels found in healthy individuals.

  • Czech name

    Analýza 8-hydroxy-2´-deoxyguanosinu v moči obézních pacientů metodou HPLC s elektrochemickou detekcí

  • Czech description

    Pro analýzu 8-hydroxy-2´-deoxyguanosinu (8-OHdG) v moči obézních pacientů metodou HPLC byl testován jednoduchý amperometrický detektor. Analyt byl extrahován z moči s použitím jednostupňové extrakce na pevné fázi s průměrným výtěžkem 71%. HPLC byla prováděna na C-18 analytických kolonách za izokratických podmínek. Zjištěný limit detekce 8-OHdG (3nM) byl srovnatelný s limitem dosaženým při použití komerčně dostupného amperometrického detektoru. Postup měření byl pak použit pro určení hladin 8-OHdG v moči10 obézních pacientů s výsledkem 32+/-27,1 nM, který se příliš nelišil od hodnoty zjištěné u zdravých jedinců.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CF - Physical chemistry and theoretical chemistry

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA203%2F01%2F0653" target="_blank" >GA203/01/0653: New electrochemical methods for quantitative detection of 8-hydroxy-2´-deoxyguanosine in urine</a><br>

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2004

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Analytica Chimica Acta

  • ISSN

    0003-2670

  • e-ISSN

  • Volume of the periodical

    516

  • Issue of the periodical within the volume

    -

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    4

  • Pages from-to

    107-110

  • UT code for WoS article

  • EID of the result in the Scopus database