Lipid diffusion in planar membranes investigated by fluorescence correlation spectroscopy

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F10%3A00345815" target="_blank" >RIV/61388955:_____/10:00345815 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Lipid diffusion in planar membranes investigated by fluorescence correlation spectroscopy
Original language description
Investigation of lipid lateral mobility in biological membranes and their artificial models provides information on membrane dynamics and structure: methods based on optical microscopy are very convenient for such investigations. We focus on fluorescencecorrelation spectroscopy (FCS), explain its principles and review its state of the art versions such as 2-focus. Z-scan or scanning FCS, which overcome most artefacts of standard FCS (especially those resulting from the need for an external calibration)making it a reliable and versatile method. FCS is also compared to single particle tracking and fluorescence photobleaching recovery and the applicability and the limitations of the methods are briefly reviewed. We discuss several key questions of lateral mobility investigation in planar lipid membranes, namely the influence which membrane and aqueous phase composition (ionic strength and sugar content), choice of a fluorescent tracer molecule, ...
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CF - Physical chemistry and theoretical chemistry
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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