Spectral analysis of doxorubicin accumulation and the indirect quantification of its DNA intercalation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F10%3A00352888" target="_blank" >RIV/61388955:_____/10:00352888 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/10:00352888 RIV/61389013:_____/10:00352888

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Spectral analysis of doxorubicin accumulation and the indirect quantification of its DNA intercalation

Original language description

We define two microscopy techniques enabling to distinguish and separate Dox and D* emission based either on its spectral properties or on fluorescence lifetime analysis. To analyze influx and nuclear accumulation of Dox (free or polymer-bound) by flow cytometry, we propose using an indirect method based on its DNA intercalation competition with Hoechst 33342 rather than a direct measurement od doxorubicin fluorescence inside the cells

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EC - Immunology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

European Journal of Pharmaceutics and Biopharmaceutics

ISSN

0939-6411

e-ISSN

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Volume of the periodical

76

Issue of the periodical within the volume

3

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

11

Pages from-to

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UT code for WoS article

000285279900027

EID of the result in the Scopus database

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