Tryptophan-Accelerated Electron Flow Across a ProteinProtein Interface

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F13%3A00397877" target="_blank" >RIV/61388955:_____/13:00397877 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1021/ja406830d" target="_blank" >http://dx.doi.org/10.1021/ja406830d</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/ja406830d" target="_blank" >10.1021/ja406830d</a>

Result language
angličtina
Original language name
Tryptophan-Accelerated Electron Flow Across a ProteinProtein Interface
Original language description
We report a new metallolabeled blue copper protein, Re126W122CuI Pseudomonas aeruginosa azurin, which has three redox sites at well-defined distances in the protein fold: ReI(CO)3(4,7-dimethyl- 1,10-phenanthroline) covalently bound at H126, a Cu center,and an indole side chain W122 situated between the Re and Cu sites (Re- W122(indole) = 13.1 ?, dmp-W122(indole) = 10.0 ?, Re-Cu = 25.6 ?). Near-UV excitation of the Re chromophore leads to prompt CuI oxidation (<50 ns), followed by slow back ET to regenerate CuI and ground-state ReI with biexponential kinetics, 220 ns and 6 ?s. From spectroscopic measurements of kinetics and relative ET yields at different concentrations, it is likely that the photoinduced ET reactions occur in protein dimers, (Re126W122CuI)2 and that the forward ET is accelerated by intermolecular electron hopping through the interfacial tryptophan: *Re//<-W122<-CuI, where // denotes a proteinprotein interface. Solution mass spectrometry confirms a broad oligomer distr
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CF - Physical chemistry and theoretical chemistry
OECD FORD branch
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Project
<a href="/en/project/LH13015" target="_blank" >LH13015: Metalloprotein photoactivation: Structural dynamics and electron transfer</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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