Time-Resolved Fluorescence in Lipid Bilayers: Selected Applications and Advantages over Steady State

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F14%3A00436040" target="_blank" >RIV/61388955:_____/14:00436040 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.bpj.2014.10.058" target="_blank" >http://dx.doi.org/10.1016/j.bpj.2014.10.058</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bpj.2014.10.058" target="_blank" >10.1016/j.bpj.2014.10.058</a>

Result language

angličtina

Original language name

Time-Resolved Fluorescence in Lipid Bilayers: Selected Applications and Advantages over Steady State

Original language description

Fluorescence methods are versatile tools for obtaining dynamic and topological information about biomembranes because the molecular interactions taking place in lipid membranes frequently occur on the same timescale as fluorescence emission. The fluorescence intensity decay, in particular, is a powerful reporter of the molecular environment of a fluorophore. The fluorescence lifetime can be sensitive to the local polarity, hydration, viscosity, and/or presence of fluorescence quenchers/ energy acceptorswithin several nanometers of the vicinity of a fluorophore. Illustrative examples of how time-resolved fluorescence measurements can provide more valuable and detailed information about a system than the time-integrated (steady-state) approach will be presented in this review: 1), determination of membrane polarity and mobility using time-dependent spectral shifts; 2), identification of submicroscopic domains by fluorescence lifetime imaging microscopy; 3), elucidation of membrane leaka

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CF - Physical chemistry and theoretical chemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biophysical Journal

ISSN

0006-3495

e-ISSN

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Volume of the periodical

107

Issue of the periodical within the volume

12

Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

2751-2760

UT code for WoS article

000346434200007

EID of the result in the Scopus database

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