High Efficacy but Low Potency of delta-Opioid Receptor-G Protein Coupling in Brij-58-Treated, Low-Density Plasma Membrane Fragments
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F15%3A00447525" target="_blank" >RIV/61388955:_____/15:00447525 - isvavai.cz</a>
Alternative codes found
RIV/67985823:_____/15:00447525
Result on the web
<a href="http://dx.doi.org/10.1371/journal.pone.0135664" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0135664</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pone.0135664" target="_blank" >10.1371/journal.pone.0135664</a>
Alternative languages
Result language
angličtina
Original language name
High Efficacy but Low Potency of delta-Opioid Receptor-G Protein Coupling in Brij-58-Treated, Low-Density Plasma Membrane Fragments
Original language description
HEK293 cells expressing DOR-Gi1alpha fusion protein were treated with non-ionic detergent Brij-58 and fractionated by flotation in sucrose density gradient. In optimum range of detergent concentrations, Brij58-treated, low-density membrane fragments exhibited 2-3-fold higher efficacy of agonist-induced G protein activity than membranes prepared in the absence of detergent. The potency of agonist response was significantly decreased. At high detergent concentrations, the functional coupling between delta-opioid receptors and G proteins was completely diminished
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CF - Physical chemistry and theoretical chemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GAP207%2F12%2F0919" target="_blank" >GAP207/12/0919: The role of hydrophobic plasma membrane interior in DOR regulation of trimeric G protein activity</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PLoS ONE
ISSN
1932-6203
e-ISSN
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Volume of the periodical
10
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
32
Pages from-to
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UT code for WoS article
000359666100055
EID of the result in the Scopus database
2-s2.0-84942436628