The effect of detergents on trimeric G-protein activity in isolated plasma membranes from rat brain cortex: Correlation with studies of DPH and Laurdan fluorescence

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F09%3A00333814" target="_blank" >RIV/61388955:_____/09:00333814 - isvavai.cz</a>
Alternative codes found
RIV/67985823:_____/09:00333814
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
The effect of detergents on trimeric G-protein activity in isolated plasma membranes from rat brain cortex: Correlation with studies of DPH and Laurdan fluorescence
Original language description
The effect of non-ionic detergents on baclofen (GABA(B)-R agonist)-stimulated G-protein activity was measured as a [S-35] GTP gamma S,IS binding assay in the plasma membranes (PM) isolated from the brain tissue. The effect was clearly biphasic - a decrease in the activity was followed by an activation maximum and finally, at high concentrations, drastic inhibition of the G-protein activity was noticed. Contrarily, specific radioligand binding to GABA(B)-receptor was inhibited in the whole range of detergent concentrations step by step, i.e. it was strictly monophasic. The magnitude of both detergent effects was decreased in the same order of potency: Brij58>Triton X-100>Digitonin. The identical order was found when comparing detergents ability to alterfluorescence anisotropy of the membrane probe 1,6-diphenyl-1,3,5-hexatriene (r (DPH)) incorporated into the hydrophobic PM interior.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CF - Physical chemistry and theoretical chemistry
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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