Anionic hexadeca-carboxylate tetrapyrazinoporphyrazine: synthesis and in vitro photodynamic studies of a water-soluble, non-aggregating photosensitizer
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F16%3A00455793" target="_blank" >RIV/61388955:_____/16:00455793 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11160/16:10326175
Result on the web
<a href="http://dx.doi.org/10.1039/C5RA25881B" target="_blank" >http://dx.doi.org/10.1039/C5RA25881B</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/C5RA25881B" target="_blank" >10.1039/C5RA25881B</a>
Alternative languages
Result language
angličtina
Original language name
Anionic hexadeca-carboxylate tetrapyrazinoporphyrazine: synthesis and in vitro photodynamic studies of a water-soluble, non-aggregating photosensitizer
Original language description
A sodium salt of zinc tetrapyrazinoporphyrazine bearing eight 3,5-dicarboxylatophenyl substituents (1) was synthesized. The presence of sixteen negative charges in a rigid arrangement on the periphery of the macrocycle inhibited its aggregation in water or buffers at pH > 5.8. Strong aggregation was, however, observed in buffers at pH < 4.8 due to the protonation of carboxylate functions. Fluorescence microscopy revealed that the compound localized to lysosomes and endosomes in cells. The compound's photodynamic activity on HeLa cancer cells (IC50 = 5.7 1.1 μM) was found to be influenced by both pH and interactions with serum proteins. This was demonstrated with a detailed in vitro study based on the inhibition of vacuolar H+-ATPase using bafilomycin A1, which increased the intracellular fluorescence of 1. Compound 1 also formed interactions with serum proteins that partially quenched its excited states; however, they also protected the compound from self-aggregation at low pH.n
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CF - Physical chemistry and theoretical chemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA13-27761S" target="_blank" >GA13-27761S: Development of new photosensitizers for photodynamic therapy and investigation of their mechanism of action on cellular level</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
RSC Advances
ISSN
2046-2069
e-ISSN
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Volume of the periodical
6
Issue of the periodical within the volume
JAN 2016
Country of publishing house
GB - UNITED KINGDOM
Number of pages
14
Pages from-to
10064-10077
UT code for WoS article
000369516100083
EID of the result in the Scopus database
2-s2.0-84961316840