Effect of bovine serum albumin on the photodynamic activity of sulfonated tetrapyrazinoporphyrazine

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F19%3A00499171" target="_blank" >RIV/61388955:_____/19:00499171 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11160/19:10399468
Result on the web
<a href="http://hdl.handle.net/11104/0291428" target="_blank" >http://hdl.handle.net/11104/0291428</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.dyepig.2018.10.051" target="_blank" >10.1016/j.dyepig.2018.10.051</a>

Result language
angličtina
Original language name
Effect of bovine serum albumin on the photodynamic activity of sulfonated tetrapyrazinoporphyrazine
Original language description
We synthesized water-soluble zinc(II) complex of octa(p-sulfonatophenyl)tetrapyrazinoporphyrazine (TPyzPz), which is monomeric above pH 2.5 up to micromolar concentration. Significant aggregation occurs at lower pH as a consequence of losing repulsion forces after protonation of sulfonate groups. Changes in both absorption and fluorescence spectra indicated strong interaction of TPyzPz and bovine serum albumin (BSA) with a maximum at 3:1 ratio (dye: BSA). Binding to the BSA leads to significant quenching of the singlet excited state of the photosensitizer and to longer triplet states lifetimes as a consequence of limited diffusion of oxygen to the excited TPyzPz. The photodynamic activity of this TPyzPz was also strongly influenced by binding to BSA. It was lower in the cell culture medium containing serum (EC50 = 99.8 ± 23.5 μM) than in the medium without the proteins (EC50 = 0.83 ± 0.31 μM). The compound was of minimal dark toxicity without irradiation (TC50 > 1000 μM). Fluorescence microscopy revealed lysosomes as the localization site of the TPyzPz, their rupture after irradiation and subsequent relocalization of TPyzPz to the cytoplasm with predominantly necrotic mode of cell death.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10403 - Physical chemistry

Project
<a href="/en/project/EF16_019%2F0000841" target="_blank" >EF16_019/0000841: Efficiency and safety improvement of current drugs and nutraceuticals: advanced methods - new challenges</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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