Interaction of lysozyme with a tear film lipid layer model: A molecular dynamics simulation study

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F17%3A00506619" target="_blank" >RIV/61388955:_____/17:00506619 - isvavai.cz</a>

Result on the web

<a href="http://hdl.handle.net/11104/0297830" target="_blank" >http://hdl.handle.net/11104/0297830</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bbamem.2017.08.015" target="_blank" >10.1016/j.bbamem.2017.08.015</a>

Result language

angličtina

Original language name

Interaction of lysozyme with a tear film lipid layer model: A molecular dynamics simulation study

Original language description

The tear film is a thin multilayered structure covering the cornea. Its outermost layer is a lipid film underneath of which resides on an aqueous layer. This tear film lipid layer (TFLL) is itself a complex structure, formed by both polar and nonpolar lipids. It was recently suggested that due to tear film dynamics, TFLL contains in homogeneities in the form of polar lipid aggregates. The aqueous phase of tear film contains lachrymal-origin proteins, whereby lysozyme is the most abundant. These proteins can alter TFLL properties, mainly by reducing its surface tension. However, a detailed nature of protein-lipid interactions in tear film is not known. We investigate the interactions of lysozyme with TFLL in molecular details by employing coarse-grained molecular dynamics simulations. We demonstrate that lysozyme, due to lateral restructuring of TFLL, is able to penetrate the tear lipid film embedded in inverse micellar aggregates.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10403 - Physical chemistry

Project

<a href="/en/project/GA15-14292S" target="_blank" >GA15-14292S: Investigation of the tear film lipid layer at the molecular level – molecular dynamics simulations and experiments employing model lipid films</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biochimica Et Biophysica Acta-Biomembranes

ISSN

0005-2736

e-ISSN

—

Volume of the periodical

1859

Issue of the periodical within the volume

12

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

2289-2296

UT code for WoS article

000415770900002

EID of the result in the Scopus database

2-s2.0-85029666406