Interactions of polar lipids with cholesteryl ester multilayers elucidate tear film lipid layer structure

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F20%3A00531105" target="_blank" >RIV/61388955:_____/20:00531105 - isvavai.cz</a>

Result on the web

<a href="http://hdl.handle.net/11104/0309842" target="_blank" >http://hdl.handle.net/11104/0309842</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jtos.2020.06.001" target="_blank" >10.1016/j.jtos.2020.06.001</a>

Result language

angličtina

Original language name

Interactions of polar lipids with cholesteryl ester multilayers elucidate tear film lipid layer structure

Original language description

Purpose: The tear film lipid layer (TFLL) covers the tear film, stabilizing it and providing a protective barrier against the environment. The TFLL is divided into polar and non-polar sublayers, but the interplay between lipid classes in these sublayers and the structure-function relationship of the TFLL remains poorly characterized. This study aims to provide insight into TFLL function by elucidating the interactions between polar and non-polar TFLL lipids at the molecular level. Methods: Mixed films of polar O-acyl-ω-hydroxy fatty acids (OAHFA) or phospholipids and non-polar cholesteryl esters (CE) were used as a model of the TFLL. The organization of the films was studied by using a combination of Brewster angle and fluorescence microscopy in a Langmuir trough system. In addition, the evaporation resistance of the lipid films was evaluated. Results: Phospholipids and OAHFAs induced the formation of a stable multilamellar CE film. The formation of this film was driven by the interdigitation of acyl chains between the monolayer of polar lipids and the CE multilayer lamellae. Surprisingly, the multilayer structure was destabilized by both low and high concentrations of polar lipids. In addition, the CE multilayer was no more effective in resisting the evaporation of water than a polar lipid monolayer. Conclusions: Formation of multilamellar films by major tear film lipids suggest that the TFLL may have a similar structure. Moreover, in contrast to the current understanding, polar TFLL lipids may not mainly act by stabilizing the non-polar TFLL sublayer, but through a direct evaporation resistant effect. © 2020 The Authors

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10403 - Physical chemistry

Project

<a href="/en/project/GA18-26751S" target="_blank" >GA18-26751S: Penetration, accumulation and interactions of selected drug-model molecules with mimics of human Tear Film Lipid Layer</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Ocular Surface

ISSN

1542-0124

e-ISSN

—

Volume of the periodical

18

Issue of the periodical within the volume

4

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

545-553

UT code for WoS article

000591804800002

EID of the result in the Scopus database

2-s2.0-85086772309