Upregulation of SPS100 gene expression by an antisense RNA via a switch of mRNA isoforms with different stabilities

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F17%3A00506620" target="_blank" >RIV/61388955:_____/17:00506620 - isvavai.cz</a>

Result on the web

<a href="http://hdl.handle.net/11104/0297831" target="_blank" >http://hdl.handle.net/11104/0297831</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gkx737" target="_blank" >10.1093/nar/gkx737</a>

Result language

angličtina

Original language name

Upregulation of SPS100 gene expression by an antisense RNA via a switch of mRNA isoforms with different stabilities

Original language description

Pervasive transcription of genomes generates multiple classes of non-coding RNAs. One of these classes are stable long non-coding RNAs which overlap coding genes in antisense direction (asRNAs). The function of such asRNAs is not fully understood but several cases of antisense-dependent gene expression regulation affecting the overlapping genes have been demonstrated. Using high-throughput yeast genetics and a limited set of four growth conditions we previously reported a regulatory function for similar to 25% of asRNAs, most of which repress the expression of the sense gene. To further explore the roles of asRNAs we tested more conditions and identified 15 conditionally antisense-regulated genes, 6 of which exhibited antisense-dependent enhancement of gene expression. We focused on the sporulation-specific gene SPS100, which becomes upregulated upon entry into starvation or sporulation as a function of the antisense transcript SUT169. We demonstrate that the antisense effect is mediated by its 3 ' intergenic region (3 '-IGR) and that this regulation can be transferred to other genes. Genetic analysis revealed that SUT169 functions by changing the relative expression of SPS100 mRNA isoforms from a short and unstable transcript to a long and stable species. These results suggest a novel mechanism of antisense-dependent gene regulation via mRNA isoform switching.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10403 - Physical chemistry

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

—

Volume of the periodical

45

Issue of the periodical within the volume

19

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

11144-11158

UT code for WoS article

000414552300022

EID of the result in the Scopus database

2-s2.0-85037984799