Distinct roles of SNARE-mimicking lipopeptides during initial steps of membrane fusion
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F18%3A00495130" target="_blank" >RIV/61388955:_____/18:00495130 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1039/C8NR05730C" target="_blank" >http://dx.doi.org/10.1039/C8NR05730C</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/C8NR05730C" target="_blank" >10.1039/C8NR05730C</a>
Alternative languages
Result language
angličtina
Original language name
Distinct roles of SNARE-mimicking lipopeptides during initial steps of membrane fusion
Original language description
A model system for membrane fusion, inspired by SNARE proteins and based on two complementary lipopeptides CPnE4 and CPnK4, has been recently developed. It consists of cholesterol (C), a poly(ethylene glycol) linker (Pn) and either a cationic peptide K4 (KIAALKE)4 or an anionic peptide E4 (EIAALEK)4. In this paper, fluorescence spectroscopy is used to decipher distinct but complementary roles of these lipopeptides during early stages of membrane fusion. Molecular evidence is provided that different distances of E4 in CPnE4 and K4 in CPnK4 from the bilayer represent an important mechanism, which enables fusion. Whereas E4 is exposed to the bulk and solely promotes membrane binding of CPnK4, K4 loops back to the lipid–water interface where it fulfills two distinct roles: it initiates bilayer contact by binding to CPnE4 containing bilayers, and it initiates fusion by modulating the bilayer properties. The interaction between CPnE4 and CPnK4 is severely down-regulated by binding of K4 to the bilayer and possible only if the lipopeptides approach each other as constituents of different bilayers. When the complementary lipopeptides are localized in the same bilayer, hetero-coiling is disabled. These data provide crucial insights as to how fusion is initiated and highlight the importance of both peptides in this process.n
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10403 - Physical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nanoscale
ISSN
2040-3364
e-ISSN
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Volume of the periodical
10
Issue of the periodical within the volume
40
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
19064-19073
UT code for WoS article
000448344100020
EID of the result in the Scopus database
2-s2.0-85055080629