Behavior of the DPH fluorescence probe in membranes perturbed by drugs

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F19%3A00517124" target="_blank" >RIV/61388955:_____/19:00517124 - isvavai.cz</a>

Result on the web

<a href="http://hdl.handle.net/11104/0302414" target="_blank" >http://hdl.handle.net/11104/0302414</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chemphyslip.2019.104784" target="_blank" >10.1016/j.chemphyslip.2019.104784</a>

Result language

angličtina

Original language name

Behavior of the DPH fluorescence probe in membranes perturbed by drugs

Original language description

1,6-Diphenyl-1,3,5-hexatriene (DPH) is one of the most commonly used fluorescent probes to study dynamical and structural properties of lipid bilayers and cellular membranes via measuring steady-state or time-resolved fluorescence anisotropy. In this study, we present a limitation in the use of DPH to predict the order of lipid acyl chains when the lipid bilayer is doped with itraconazole (ITZ), an antifungal drug. Our steady-state fluorescence anisotropy measurements showed a significant decrease in fluorescence anisotropy of DPH embedded in the ITZ-containing membrane, suggesting a substantial increase in membrane fluidity, which indirectly indicates a decrease in the order of the hydrocarbon chains. This result or its interpretation is in disagreement with the fluorescence recovery after photobleaching measurements and molecular dynamics (MD) simulation data. The results of these experiments and calculations indicate an increase in the hydrocarbon chain order. The MD simulations of the bilayer containing both ITZ and DPH provide explanations for these observations. Apparently, in the presence of the drug, the DPH molecules are pushed deeper into the hydrophobic membrane core below the lipid double bonds, and the probe predominately adopts the orientation of the ITZ molecules that is parallel to the membrane surface, instead of orienting parallel to the lipid acyl chains. For this reason, DPH anisotropy provides information related to the less ordered central region of the membrane rather than reporting the properties of the upper segments of the lipid acyl chains.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10403 - Physical chemistry

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemistry and Physics of Lipids

ISSN

0009-3084

e-ISSN

—

Volume of the periodical

223

Issue of the periodical within the volume

SEP 2019

Country of publishing house

IE - IRELAND

Number of pages

9

Pages from-to

104784

UT code for WoS article

000482245400008

EID of the result in the Scopus database

2-s2.0-85067516562