Modulation of Anionic Lipid Bilayers by Specific Interplay of Protons and Calcium Ions
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F22%3A00566604" target="_blank" >RIV/61388955:_____/22:00566604 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11320/22:10455509
Result on the web
<a href="https://hdl.handle.net/11104/0337920" target="_blank" >https://hdl.handle.net/11104/0337920</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/biom12121894" target="_blank" >10.3390/biom12121894</a>
Alternative languages
Result language
angličtina
Original language name
Modulation of Anionic Lipid Bilayers by Specific Interplay of Protons and Calcium Ions
Original language description
Biomembranes, important building blocks of living organisms, are often exposed to large local fluctuations of pH and ionic strength. To capture changes in the membrane organization under such harsh conditions, we investigated the mobility and hydration of zwitterionic and anionic lipid bilayers upon elevated H3O+ and Ca2+ content by the time-dependent fluorescence shift (TDFS) technique. While the zwitterionic bilayers remain inert to lower pH and increased calcium concentrations, anionic membranes are responsive. Specifically, both bilayers enriched in phosphatidylserine (PS) and phosphatidylglycerol (PG) become dehydrated and rigidified at pH 4.0 compared to at pH 7.0. However, their reaction to the gradual Ca2+ increase in the acidic environment differs. While the PG bilayers exhibit strong rehydration and mild loosening of the carbonyl region, restoring membrane properties to those observed at pH 7.0, the PS bilayers remain dehydrated with minor bilayer stiffening. Molecular dynamics (MD) simulations support the strong binding of H3O+ to both PS and PG. Compared to PS, PG exhibits a weaker binding of Ca2+ also at a low pH.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10403 - Physical chemistry
Result continuities
Project
<a href="/en/project/GA22-25953S" target="_blank" >GA22-25953S: Graphene-Induced Energy Transfer Spectroscopy of tethered lipid bilayers</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biomolecules
ISSN
2218-273X
e-ISSN
2218-273X
Volume of the periodical
12
Issue of the periodical within the volume
12
Country of publishing house
CH - SWITZERLAND
Number of pages
13
Pages from-to
1894
UT code for WoS article
000902282700001
EID of the result in the Scopus database
2-s2.0-85144710558