Interleaflet organization of membrane nanodomains: What can(not) be resolved by FRET?

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F23%3A00572784" target="_blank" >RIV/61388955:_____/23:00572784 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/23:10451120 RIV/00216208:11320/23:10451120
Result on the web
<a href="https://hdl.handle.net/11104/0343351" target="_blank" >https://hdl.handle.net/11104/0343351</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bpj.2022.11.014" target="_blank" >10.1016/j.bpj.2022.11.014</a>

Result language
angličtina
Original language name
Interleaflet organization of membrane nanodomains: What can(not) be resolved by FRET?
Original language description
Plasma membranes as well as their simplified model systems show an inherent nanoscale heterogeneity. As a result of strong interleaflet interactions, these nanoheterogeneities (called here lipid nanodomains) can be found in perfect registration (i.e., nanodomains in the inner leaflet are registered with the nanodomains in the outer leaflet). Alternatively, they might be interleaflet independent, antiregistered, or located asymmetrically in one bilayer leaflet only. To distinguish these scenarios from each other appears to be an experimental challenge. In this work, we analyzed the potential of Förster resonance energy transfer to characterize interleaflet organization of nanodomains. We generated in silico time-resolved fluorescence decays for a large set of virtual as well as real donor/acceptor pairs distributed over the bilayer containing registered, independent, antiregistered, or asymmetrically distributed nanodomains. In this way, we were able to identify conditions that gave satisfactory or unsatisfactory resolution. Overall, Förster resonance energy transfer appears as a robust method that, when using donor/acceptor pairs with good characteristics, yields otherwise difficult-to-reach characteristics of membrane lipid nanodomains.
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10403 - Physical chemistry

Project
<a href="/en/project/GC20-01401J" target="_blank" >GC20-01401J: Exploring the structure function relationship of membrane-pore-forming FGF2 oligomers - a single molecule approach</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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