Limitations of Electronic Energy Transfer in the Determination of Lipid Nanodomain Sizes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F11%3A00369083" target="_blank" >RIV/61388955:_____/11:00369083 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.bpj.2011.11.001" target="_blank" >http://dx.doi.org/10.1016/j.bpj.2011.11.001</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bpj.2011.11.001" target="_blank" >10.1016/j.bpj.2011.11.001</a>
Alternative languages
Result language
angličtina
Original language name
Limitations of Electronic Energy Transfer in the Determination of Lipid Nanodomain Sizes
Original language description
Even though superresolution microscopy indicates that size of plasma membrane rafts is <20 nm, those structures have never been observed. Förster resonance energy transfer (FRET) is therefore still the most powerful optical method for characterization ofsuch domains. In this letter we investigate relation between nanodomain affinity of a donor-acceptor (D/A) pair and the detectable nanodomain size/area. We show that probes with high affinity to the liquid-ordered (Lo) phase are required for detecting domain sizes of a few nanometers, and/or domains that occupy a few percent of the bilayer area. A combination of donors and acceptors that prefer different phases is the more favorable approach. For instance, a D/A pair with the distribution constant of donors KD = 5 and acceptors KA = 0.01 can resolve a broad spectrum of nanodomain sizes. On the other hand, currently available donors and acceptors that prefer the same phase, either the liquid-disordered (Ld) or Lo phase, are not so conve
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CF - Physical chemistry and theoretical chemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biophysical Journal
ISSN
0006-3495
e-ISSN
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Volume of the periodical
101
Issue of the periodical within the volume
11
Country of publishing house
US - UNITED STATES
Number of pages
3
Pages from-to
"L60"-"L62"
UT code for WoS article
000297897300002
EID of the result in the Scopus database
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