Structural characterization of wax esters using ultraviolet photodissociation mass spectrometry

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F24%3A00587978" target="_blank" >RIV/61388955:_____/24:00587978 - isvavai.cz</a>

Alternative codes found

RIV/61388963:_____/24:00587978 RIV/00216208:11310/24:10493019

Result on the web

<a href="https://doi.org/10.1007/s00216-024-05434-2" target="_blank" >https://doi.org/10.1007/s00216-024-05434-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00216-024-05434-2" target="_blank" >10.1007/s00216-024-05434-2</a>

Result language

angličtina

Original language name

Structural characterization of wax esters using ultraviolet photodissociation mass spectrometry

Original language description

Wax esters play critical roles in biological systems, serving functions from energy storage to chemical signaling. Their diversity is attributed to variations in alcohol and acyl chains, including their length, branching, and the stereochemistry of double bonds. Traditional analysis by mass spectrometry with collisional activations (CID, HCD) offers insights into acyl chain lengths and unsaturation level. Still, it falls short in pinpointing more nuanced structural features like the position of double bonds. As a solution, this study explores the application of 213-nm ultraviolet photodissociation (UVPD) for the detailed structural analysis of wax esters. It is shown that lithium adducts provide unique fragments as a result of Norrish and Norrish-Yang reactions at the ester moieties and photoinduced cleavages of double bonds. The product ions are useful for determining chain lengths and localizing double bonds. UVPD spectra of various wax esters are presented systematically, and the effect of activation time is discussed. The applicability of tandem mass spectrometry with UVPD is demonstrated for wax esters from natural sources. The UHPLC analysis of jojoba oil proves the compatibility of MS2 UVPD with the chromatography time scale, and a direct infusion is used to analyze wax esters from vernix caseosa. Data shows the potential of UVPD and its combination with CID or HCD in advancing our understanding of wax ester structures.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10403 - Physical chemistry

Project

<a href="/en/project/LX22NPO5104" target="_blank" >LX22NPO5104: National Institute for Research of Metabolic and Cardiovascular Diseases</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical and Bioanalytical Chemistry

ISSN

1618-2642

e-ISSN

1618-2650

Volume of the periodical

416

Issue of the periodical within the volume

25

Country of publishing house

DE - GERMANY

Number of pages

16

Pages from-to

5497-5512

UT code for WoS article

001272308900001

EID of the result in the Scopus database

2-s2.0-85198954455