Can calmodulin bind to lipids of the cytosolic leaflet of plasma membranes?
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F24%3A00598610" target="_blank" >RIV/61388955:_____/24:00598610 - isvavai.cz</a>
Alternative codes found
RIV/61388963:_____/24:00598610 RIV/60076658:12310/24:43908812 RIV/00216224:90127/24:00138505
Result on the web
<a href="https://royalsocietypublishing.org/doi/10.1098/rsob.240067" target="_blank" >https://royalsocietypublishing.org/doi/10.1098/rsob.240067</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1098/rsob.240067" target="_blank" >10.1098/rsob.240067</a>
Alternative languages
Result language
angličtina
Original language name
Can calmodulin bind to lipids of the cytosolic leaflet of plasma membranes?
Original language description
Calmodulin (CaM) is a ubiquitous calcium-sensitive messenger in eukaryotic cells. It was previously shown that CaM possesses an affinity for diverse lipid moieties, including those found on CaM-binding proteins. These facts, together with our observation that CaM accumulates in membrane-rich protrusions of HeLa cells upon increased cytosolic calcium, motivated us to perform a systematic search for unmediated CaM interactions with model lipid membranes mimicking the cytosolic leaflet of plasma membranes. A range of experimental techniques and molecular dynamics simulations prove unambiguously that CaM interacts with lipid bilayers in the presence of calcium ions. The lipids phosphatidylserine (PS) and phosphatidylethanolamine (PE) hold the key to CaM-membrane interactions. Calcium induces an essential conformational rearrangement of CaM, but calcium binding to the headgroup of PS also neutralizes the membrane negative surface charge. More intriguingly, PE plays a dual role-it not only forms hydrogen bonds with CaM, but also destabilizes the lipid bilayer increasing the exposure of hydrophobic acyl chains to the interacting proteins. Our findings suggest that upon increased intracellular calcium concentration, CaM and the cytosolic leaflet of cellular membranes can be functionally connected.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10403 - Physical chemistry
Result continuities
Project
<a href="/en/project/GX19-26854X" target="_blank" >GX19-26854X: Concert of lipids, ions, and proteins in cell membrane dynamics and function</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Open Biology
ISSN
2046-2441
e-ISSN
2046-2441
Volume of the periodical
14
Issue of the periodical within the volume
9
Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
240067
UT code for WoS article
001313871600001
EID of the result in the Scopus database
2-s2.0-85204512599