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Cell surface morphology mimicking nano-bio platform for immune cell stimulation

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F24%3A00600622" target="_blank" >RIV/61388955:_____/24:00600622 - isvavai.cz</a>

  • Alternative codes found

    RIV/60461373:22310/24:43930389

  • Result on the web

    <a href="https://hdl.handle.net/11104/0357936" target="_blank" >https://hdl.handle.net/11104/0357936</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.isci.2024.111033" target="_blank" >10.1016/j.isci.2024.111033</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Cell surface morphology mimicking nano-bio platform for immune cell stimulation

  • Original language description

    Studying the complex realm of cellular communication and interactions by fluorescence microscopy requires sample fixation on a transparent substrate. To activate T cells, which are pivotal in controlling the immune system, it is important to present the activating antigen in a spatial arrangement similar to the nature of the antigen-presenting cell, including the presence of ligands on microvilli. Similar arrangement is predicted for some other immune cells. In this work, immune cell-stimulating platform based on nanoparticle-ligand conjugates have been developed using a scalable, affordable, and broadly applicable technology, which can be readily deployed without the need for state-of-the-art nanofabrication instruments. The validation of surface biofunctionalization was performed by combination of fluorescence and atomic force microscopy techniques. We demonstrate that the targeted system serves as a biomimetic scaffold on which immune cells make primary contact with the microvilli-mimicking substrate and exhibit stimulus-specific activation.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10403 - Physical chemistry

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2024

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    iScience

  • ISSN

    2589-0042

  • e-ISSN

    2589-0042

  • Volume of the periodical

    27

  • Issue of the periodical within the volume

    11

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    10

  • Pages from-to

    111033

  • UT code for WoS article

    001341080900001

  • EID of the result in the Scopus database

    2-s2.0-85207803981