Revised Diffusion Law Permits Quantitative Nanoscale Characterization of Membrane Organization

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F25%3A00635958" target="_blank" >RIV/61388955:_____/25:00635958 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/25:10502245 RIV/00216208:11320/25:10502245
Result on the web
<a href="https://hdl.handle.net/11104/0366964" target="_blank" >https://hdl.handle.net/11104/0366964</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.analchem.5c00021" target="_blank" >10.1021/acs.analchem.5c00021</a>

Result language
angličtina
Original language name
Revised Diffusion Law Permits Quantitative Nanoscale Characterization of Membrane Organization
Original language description
The formation of functional nanoscopic domains is an inherent property of plasma membranes. Stimulated emission depletion combined with fluorescence correlation spectroscopy (STED-FCS) has been previously used to identify such domains. However, the information obtained by STED-FCS has been limited to the presence of such domains while crucial parameters have not been accessible, such as size (R d), the fraction of occupied membrane surface (f), in-membrane lipid diffusion inside (D in) and outside (D out) the nanodomains as well as their self-diffusion (D d). Here, we introduce a quantitative approach based on a revised interpretation of the diffusion law. By analyzing experimentally recorded STED-FCS diffusion law plots using a comprehensive library of simulated diffusion law plots, we extract these five parameters from STED-FCS data. That approach is verified on ganglioside nanodomains in giant unilamellar vesicles, validating the Saffman-Delbruck assumption for D d. STED-FCS data in both plasma membranes of living PtK2 cells and giant plasma membrane vesicles are examined, and a quantitative framework for molecular diffusion modes in biological membranes is presented.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10403 - Physical chemistry

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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