Crystallization and preliminary X-ray diffraction analysis of mouse galectin-4 N-terminal carbohydrate recognition domain in complex with lactose

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F08%3A00312444" target="_blank" >RIV/61388963:_____/08:00312444 - isvavai.cz</a>

Alternative codes found

RIV/68378050:_____/08:00312444

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Crystallization and preliminary X-ray diffraction analysis of mouse galectin-4 N-terminal carbohydrate recognition domain in complex with lactose

Original language description

Galectin-4 is thought to play a role in the process of tumour conversion of cells of the alimentary tract and the breast tissue; however, its exact function remains unknown. With the aim of elucidating the structural basis of mouse galectin-4 (mGal-4) binding specificity, we have undertaken X-ray analysis of the N-terminal domain, CRD1, of mGal-4 in complex with lactose (the basic building block of known galectin-4 carbohydrate ligands). An optimized crystallization procedure and cryocooling protocol allowed us to extend resolution to 2.1 A. Structure refinement is currently under way; the initial electron-density maps clearly show non-protein electron density in the vicinity of the carbohydrate binding site, indicating the presence of one lactose molecule. The structure will help to improve understanding of the binding specificity and function of the potential colon cancer marker galectin-4.

Czech name

Krystalizace a předběžná rentgenostrukturní analýza komplexu N-koncové CRD domény myšího galektinu-4 s laktosou

Czech description

Galektin-4 zřejmě hraje roli v nádorové konverzi buněk trávicího traktu, jeho přesná funkce však dosud není známa. Cílem práce bylo přispět k objasnění strukturního základu vazebné specifity myšího galektinu-4 pomocí rentgenostrukturní analýzy N-koncové,cukr rozpoznávající domény CRD1, v komplexu s laktosou. Struktura přispěje k pochopení vazebné specifity a funkce galektinu-4, potenciálního markeru nádorů tlustého střeva.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA304%2F03%2F0090" target="_blank" >GA304/03/0090: Studies of the mouse galectin-4 and its recombinant CRD domains in relation to the binding of sacharides and recognition of cell ligands</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Crystallographica Section F

ISSN

1744-3091

e-ISSN

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Volume of the periodical

64

Issue of the periodical within the volume

7

Country of publishing house

GB - UNITED KINGDOM

Number of pages

3

Pages from-to

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UT code for WoS article

000257249000026

EID of the result in the Scopus database

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