Role of the carbohydrate recognition domains of mouse galectin-4 in oligosaccharide binding and epitope recognition and expression of galectin-4 and galectin-6 in mouse cells and tissues

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F06%3A00043116" target="_blank" >RIV/68378050:_____/06:00043116 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/06:00004898

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Role of the carbohydrate recognition domains of mouse galectin-4 in oligosaccharide binding and epitope recognition and expression of galectin-4 and galectin-6 in mouse cells and tissues

Original language description

Galectin-4 and galectin-6 found in the mouse are closely related tandem-repeat members of the galectin family. We studied the expression of both galectins in mouse tissues, cell lines and early mouse embryogenesis. To study the role of carbohydrate recognition domains (CRDs) in oligosaccharide binding and epitope recognition, we cloned mouse full-length galectin-4 and galectin-6 cDNA and constructed bacterial expression vectors producing histidine-tagged recombinant galectin-4 and its truncated CRD1 andCRD2 forms. Preparation of these recombinant forms of mouse galectin-4 and its CRD domains allowed us to investigate the oligosaccharide binding profile using Glycan Array analysis of 185 different glycan structures.

Czech name

Úloha uhlovodíkových rekogničních domén myšího galektinu-4 ve vazbě oligosacharidů a rozpoznávání epitopů a exprese galektinu-4 a galektinu-6 v myších buňkách a tkáních

Czech description

Studovali jsme expresi galektinu-4 a galektinu-6 v myších tkáních a bunečných liniích. Připravili jsme rekombinantní CRD domény galektinu-4 a galektinu-6 s histidinovou kotvou a dále studovali vazbu oligosacharidů na CRD domény galektinu-4 pomocí Glycanarray analýzy.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

—

Project

<a href="/en/project/GA304%2F03%2F0090" target="_blank" >GA304/03/0090: Studies of the mouse galectin-4 and its recombinant CRD domains in relation to the binding of sacharides and recognition of cell ligands</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Molecular Medicine

ISSN

1107-3756

e-ISSN

—

Volume of the periodical

18

Issue of the periodical within the volume

1

Country of publishing house

GR - GREECE

Number of pages

12

Pages from-to

65-76

UT code for WoS article

—

EID of the result in the Scopus database

—